Alert® for Histamine

Alert® for Histamine is a competitive direct ELISA intended for the screening of histamine in scombroid species of fish, such as tuna, bluefish and mahi-mahi, and in fishmeal.

Specifications
Brand	Alert®
Analyte	Histamine
Platform	ELISA
Result Type	Qualitative
Limit of Detection	50 ppm
Testing Time	20.00
Cross Reactivity	Specific for histamine. No cross-reactivity with other biogenic amines.
Quantity per Package	20 tests
Package Weight	1.30 lb

Categories: Alert®, Histamine

Description
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Description

Alert® for Histamine

SKU No. 9515

Simple water extraction
20 minutes to results
Test one or up to five samples at one time

Materials Provided

24 antibody-coated microwells
24 red-marked mixing wells
1 yellow-labeled bottle of 50 ppm histamine control
1 blue-labeled bottle of histamine-HRP conjugate solution
20 bottles of sample extract diluent buffer
1 bottle of 80 mL wash buffer
1 green-labeled bottle of K-Blue^® Substrate solution
1 red-labeled bottle of Red Stop solution
12 disposable beakers

Materials Required But Not Provided

Alert for Histamine extraction kit (Neogen item^® 9520) — contains 20 bottles, 20 filter syringes and 20 tubes
Filter syringes for histamine (Neogen item 9420H), Whatman no. 1 filter paper (Neogen item 9430), centrifuge or equivalent
Tubes with caps, 5 mL (Neogen items 9421, 9421B)
Graduated cylinder
Blender
Scale capable of weighing 10-50 grams (Neogen item 9427)
Pipettor, 100 µL (Neogen items 9272, 9290)
Pipette tips (Neogen items 9410, 9407, 9417)
Paper towels or equivalent absorbent material
Microwell holder (Neogen item 9402)
Timer (Neogen item 9426)
Waterproof marker
Wash bottle (Neogen item 9400)
Distilled or deionized water